Diagnosis of an Infected Patient Essay Essay

As we know bacteria comes in various classes and are distinguished by their shapes, chemical configuration, source of energy either sunlight or chemicals, nutritional requisites and biochemical actions. We have a patient that may be infected with an unknown bacterial infection; we’ve collected a sputum sample and will be testing for one of the following genera: Bacillus, Escherichia and Mycoplasma. In order to determine which one of the three may be the source of the infection we have to recognize the characteristics of each one and the staining protocol needed to identify them. All three of the above mentioned genera are considered to be members of the Prokaryotic cell family which includes bacteria. They have certain identifiable features that distinguishes them from Eukaryotes such as: â€Å"Their DNA is not enclosed within a membrane† and â€Å"they usually divide by binary fusion, organelles are not encompassed within the membrane and the cell walls usually consis t of complex polysaccharides peptidoglycan† (Tortora, 2013). When researching the differences among the three genera’s each have specific characteristics that distinguishes one from another; Mycoplasma are the smallest members of the prokaryote family, due to their size they lack cell walls or have a scant amount of wall material, but they are known to thrive and reproduce outside the living host cells. The plasma membranes of this bacteria contain lipids (sterols) to protect them from lysis (Tortora, 2013). Bacillus are rod shaped bacteria that are usually presented as singular (single bacilli); however when they divide into pairs they’re called Diplobacilli and when appearing as a chain it’s known as streptobacilli; there’s also bacillus that resemble cocci and are referred to as cocobacilli. Escherichia also known as E. coli is a rod shaped bacteria consisting of â€Å"flagellar protein† known as H- antigens to help differentiate the various servoar within the E. coli strain. In order to continue with our investigation as to which genera is causing the infection we must determine the appropriate staining method for each bacteria listed above. Let’s begin with Mycoplasma because this genera has a very thin or lacks a cell wall we will consider this a â€Å"Gram- positive† bacteria. If mycoplasma retains its structure, size and capsules that’s an indication of  the type of bacteria causing the infection in the patient. Bacillus are part of the prokaryotic cell family containing a thin layer of peptidoglycan; however they are spore forming; this trait helps to identify them as â€Å"gram-positive†. Step (1) in the staining process is adding the sputum to the slide, step (2) is applying heat to the sample, step (3) adding crystal-violet dye which turns the cytoplasm cells purple and applying mordant to the specimen, step (4) wash with de-coloring solution (alcohol) which causes the peptidoglycan to become more impermeable crystal –violet and iodine, step (5) counterstain is absorbed and is masked by the primary purple dye that was previously absorbed by the â€Å"gram-positive† cell s (pg. 86). It’s during this step when the microbes are identified. While the bacillus is in the aging process some cells die and give the appearance of gram-negative bacteria; these are known as â€Å"gram-variable† (Tortora, 2013). Escherichia (E.coli) these rod shaped microorganisms have a thick outer membrane; however they are non-spore forming; therefore they are considered â€Å"gram-negative† cells. The steps in the staining of â€Å"gram-negative† cells are as follows: Step (1) in the staining process is adding the sputum to the slide, step (2) is applying heat to the sample to allow the sample to remain on the slide, step (3) adding crystal-violet dye which turns the cytoplasm cells purple then apply mordant to the specimen, step (4) wash with de-coloring solution (alcohol) which allows the crystal violet-iodine to diffuse through holes in the peptidoglycan layer of the cell, step (5) add the counterstain which turns the specimen red/pink this process helps to identify the microbes; as they lack color (Tortora, 2013). Wi thout color we would be unable to identify the microorganisms creating the infections created by â€Å"gram-negative† bacteria because they are color-less. References: Tortora, Funke, Case: Microbiology an Introduction 2013 (11th edition).

Attitudes and Behaviors Essay

So let’s start by saying that attitudes have two main components those happen to be beliefs and values. Beliefs are statements while values are judgments. When it comes to persuasion a person might have their own attitude and have it rub off so to speak onto another person or several other people. We tend to use this on a daily basis for things that we want whether that be for ourselves or from other people. When it comes to conformity many individuals will change their behaviors to fit in with other people that they involve themselves with. This could potentially mean that you could lose your own identity and become someone completely different from whom you truly are. When it comes to bias we are all wired to have judgments and prejudice many times people will use this judgment and have a set attitude on people, places and things. Only people can change their behaviors. Psychologists define attitudes as a learned tendency to view things in certain ways. This can include people. Places, issues and events that occur in a person’s life. Attitudes form from the experiences that you go through, they could direct from personal experiences or from the observation of other individuals. Attitudes can be learned in several different ways. Classical conditioning can impact your attitude to different products. Operant conditioning can also help in how attitudes are developed. When someone you like has a different attitude your attitude more than likely will change to be the same as the other individual. Researchers have come to the belief and conclusion that people behave according to their attitudes under a lot of circumstances. Cognitive dissonance is when an individual experiences, psychological stress caused by conflicts from thoughts and beliefs in a person’s everyday life whether that be from work, relationships, money problems. In order to gain back control people often change their attitudes based on actual behaviors. Attitudes can change just like people can. You can turn your thought process around just  like you can with many other things. Classical conditioning can create positive emotional reactions. Operant conditioning can be used to strengthen attitudes and behaviors that you are lacking or that are weak. People can also see how negative people act and decide that they don’t want to be that way and that can drastically change a person’s mind on how they think. Life events such as death, sickness, relationship loses and other important things can make a person realize what they have and what they need to be grateful for but also can help them understand their thought patterns were incorrect and that they need to change that in order to have a better life for not only themselves but the people that they associate themselves with. The theory of persuasion says that people can alter their personal attitudes in two ways. They can be motivated to listen and to think about what is being said to them which can make them open up to different ideas and thoughts or they could simply be influenced by good positive people that they are around on a daily basis. In order to change a individual s behavior you must change your thought process completely. http://www.examiner.com/article/bias-attitude-and-prejudice http://www.cios.org/encyclopedia/persuasion/Aintroduction_4nature.htm http://psychology.about.com/od/socialinfluence/f/conformity.htm

Gender Criticism and Frankenstein Essay Example | Topics and Well Written Essays - 1250 words

Gender Criticism and Frankenstein - Essay Example other words, the work of Mary Shelley, specifically in context to Frankenstein, can be critiqued for being male dominating and critically chauvinist in its character. The protagonist and the antagonist, both the main characters of the novel around which the story revolves, are male characters, which suggest the biased sense of character in the book. The women mentioned in the book solely perform a supportive, yet a subservient role to the picture. Moving past the characters, the themes of the text also portray gender anxieties. The obsession with nine months, for instance, which Victor takes to create the monster, clearly reflects an issue with gender complexities, more of which would further be expanded on in the paper.This implies that Shelley was of the view that though women is the weaker gender, yet her role can`t be ignored in entirety to smoothly run the system. The other supportive roles, comparatively stronger in nature have been naturally attributed to men in the story. Thi s biasness attributed to gender roles can be related in context to Shelley`s personal life, which was dominated by two male figures, her father and her husband. Victor Frankenstein is inspired after having met Walton who takes nine month to explore the North Pole. Owing to his experiments, Victor ends up creating a monster, which is the underlying theme of the novel and creates the angle of fear and anxiety in the text. The two main characters in the novel, Walton and Victor are extremely ambitious and egotistical in nature, preferring a bond with another man instead of a woman. These traits have also been found characteristic to her husband in particular, reflecting the individual impacts of men on her life. More so, a huge contrast is seen when she characterizes the women in the novel representing them as ‘proper’ women in compliance tothe expectations of the mainstream society. In this scenario, it may be concluded that Shelley had a strong impact of the trend during the time

Staffing Organization for a Gourmet Coffee Shop Assignment

Staffing Organization for a Gourmet Coffee Shop - Assignment Example I am planning to initiate a gourmet coffee shop next to a college campus in Washington, DC. During the week days, hours of operation will be from 6:00 a.m. and 10:00 p.m. and start at 7:00 a.m. to 3:00 p.m. on weekends. The coffee shop is planning to have 3 store managers and 10 coffee servers. Below is my first part of the Staffing Organization that contains ways I plan to staff and manage the business for the next 3 years, and run it profitably. 1 The type of relationship I will establish between the coffee shop and employees from a legal perspective affects the duration that I will have them in the coffee shop. I will apply the indefinite duration employment contract in recruiting workers. They will have to sign a contract that defines their existence in the shop. In this contract, their continuous offering of service will be intended to last for an indefinite time (Smith & Smith, 2012). The contract has rights and responsibility that have to be followed and adhered to, for exampl e, the right to be informed when being terminated. I believe employees will work to their perfection to avoid losing a job. The type of relationship will augment the coffee shop’s profit. I will not be subjected to pay employees fixed amount of money for duration, but for efforts put in the business (Smith & Smith, 2012). ... The employment policies that define the salaries, promotions and hiring of employment are audited frequently to find out whether they are advantageous to workers. I will make sure that all information on promotion opportunities of the company reaches every employee. I will make an effort of identifying and removing barriers that deter a leave or absence from work so that employees can relax when needed (Carroll & Buchholtz, 2010). I will create effectual communication field with employees where they can air out their issues openly to be looked at. Lastly, I will train and promote hard working staffs to a higher echelon; hence, encourage them to put more efforts in their work. 3 External factors hindering staffing of the coffee shop include the modern complex technology that requires extensive and intensive edification to operate them efficiently (Koontz & Weihrich, 2007). The public is an external factor that hinders staffing since workers are required to adhere to high ethical stand ards when dealing with the public. Legal and political constraints affect staffing of coffee shop since they are obliged to follow government laws that guide the business operation. Recruiting and selecting workers is an external factor influencing staffing since it is difficult to get hardworking workers who are profit oriented (Koontz & Weihrich, 2007). Competition is a core factor that affects staffing. I will want to have innovative workers who make business boom and became a top class coffee shop. Lastly, the trade unions affect the staffing because of their requirements they want business organization to possess. 4 The plan will involve, analyzing the current labor supply, forecasting

What are the benefits to an advertiser of TV instead of print Essay

What are the benefits to an advertiser of TV instead of print - Essay Example The three traditional media platforms — radio, print and television — in addition to the emerging powerhouse, the Internet, are all effective marketing and advertising tools. This paper will examine some of the benefits in two-way comparisons. Their respective unique benefits for advertisers are primarily anchored on their own publics - their audience, listeners and viewers. These differentiated populations will explain why one is better than the others. Of course, this variable is in addition to other factors as well, including the manner in which contents are delivered. This paper will examine some of the benefits in two-way comparisons. TV vs. Print Advertisers will prefer television than newspapers and magazines fundamentally because television can broadcast moving pictures, hence, is more effective platform to market products. According to Hansen and Christensen, television has more emotional impact as evidenced by how most people remember TV advertisements than th ose placed in other media (279). In addition, television viewership can surpass print subscriptions. Statistics show, for instance, that 99 percent of the American household own at least one television (Herr). Print readership, on the other hand, is declining in the past years. For example, newspaper subscription has dropped 8.74 percent in 2010, placing the overall readership to less than 100 million (discounting readership of their online versions) (Lefkow 2010). Advertisers are increasingly becoming aware of this and, as a response, they are now purchasing advertising space in newspapers that are decentralized and specialized, meaning to reach their intended market more effectively (Cranberg, Bezanson and Soloski, 130). The decline in readership has led to the downtrend in newspaper advertising revenue as demonstrated in the following table: Table 1: Newspaper Ad Revenue (OECD, 60) Radio and TV Although radio appears to be ineffective in advertising in comparison to other traditi onal media the case is actually the opposite. Radio holds significant advantage over TV in the eyes of advertisers. Shimp outlined these as: 1) the ability to reach segmented audiences; 2) intimacy; 3) economy; 4) short lead times; 5) easy transfer of imagery from television; and, 5) the use of local personalities (372). To explain of these advantages in detail, let us consider the "intimacy" variable. Unlike television, radio is a platform that people feel closer to because they can identify with it. Radio advertising, wrote Shimp, is a personal and intimate form of friendly persuasion that has potential to increase consumers' engagement with advertisement placement in the medium (273). Then, there is also the ease by which television content could be transformed into radio broadcast. An ad can be both aired on TV and radio without any major tweaking. Even without the visuals, consumers can identify the ad and recall what is seen previously on TV. A case in point is how Pizza Hut r elies heavily on radio to leverage its TV ad spots. For instance, Garfield, the popular cartoon character, was easily transported from TV to radio ads with his personal, entertaining and informative message sandwiched between music beds that featured Pizza Hut’s one-number jingle† (Weinberger, Campbell and Brody 1994, 133). Internet over Traditional Media? Technology is actually the biggest advantage of the Internet over each platform of the traditional media. DSL, cable, broadband, satellite, etc. - these technologies have enabled millions upon millions of users to access information at the speed and convenience never before seen. For advertisers the Internet is important because of the number of users available. More important, however, is the kind of "intelligent" advertising allowed by high-technology. For instance, softwares, applications and databases can enable advertisers to find and communicate with specific target markets. The idea is

Marketing Plan for Starbucks Research Paper Example | Topics and Well Written Essays - 1750 words

Marketing Plan for Starbucks - Research Paper Example A representative of the Oromia Coffee Farmers Cooperative Union. Tadesse Meskela argues that Starbucks sells its coffee in the market for $14 per pound whereas; it buys coffee for only $1.20 per pound which is even lower than production cost (Hot News, 2010). Such concerns are being discussed at the political and national level. Coffee producing nations can also impact the operations of the company and can threaten the United States to stop the trade of coffee. For the Ethiopian farmer's trademarking has become very important, therefore, Ethiopian government asked Starbucks to sign an agreement of ownership in Coffee, however, Starbucks refused to sign it (Oromoindex, 2009). Such concerns can directly influence the operations of a company. Increasing inflation has increased the cost of coffee, thereby, affected the revenues of coffee companies. Starbucks’ first-quarter sales increased by 2.9 percent because of higher coffee prices (Singh, 2009). The growing demand for energy and other beverages and food can influence the demand of coffee in the U.S. According to 2009 trends, consumption of coffee remained consistent at 54 percent of the total adult population partaking (National Coffee Association of USA, 2009). Discretionary spending in the U.S. for the year 2010 is $1.39 trillion and 38 percent out of total spending. Around $844 billion was spent on security and $553 billion was spent on agriculture ($25 billion) (Amadeo, 2010). Technological developments have also enhanced the capability of Starbucks to increase its green practices. For example, at several stores of Starbucks, the company is experimenting with compact fluorescent lights and energy saving fixtures (Allison, 2008). Environmental protection programs and practices are very important for the companies operating in the United States.

Brooklyn Community Assesment Research Paper Example | Topics and Well Written Essays - 1250 words

Brooklyn Community Assesment - Research Paper Example In order to gather data about the Brooklyn community, various US governmental reports and annual reports of the organizations or the agencies dealing with this particular community have been effectively utilized. Apart from these, valuable data about the community has been gathered through utilizing varied reliable sources including newspapers, websites and community magazines of the US. In order to describe the boundaries of Brooklyn community, it will be vital to mention that a huge proportion of the individuals residing within this community uneducated, which eventually imposed adverse impact on its development. Justifiably, the prevalence of low literacy rate has caused employment related problems in Brooklyn community at large. As per the report published by City of New York (2012), the prevalence of greater unemployment rate has certainly emerged the conduct of numerous violent activities in the locality of Brooklyn community (City of New York, 2012). Apart from this, it can al so be ascertained that medical science and hospital facilities are not up to satisfactory mark, which has been troubling the citizens residing within Brooklyn community. Thus, mortality rate of Brooklyn community is higher than that of the average mortality rate of the US. The report of New York City College of Technology (2013) stated that one out of three adults does not insure their life in Brooklyn community. Similarly, 31 % of the residents within Brooklyn do not get involved in having medical check-up regularly.